韓牛에 있어서 anaplama marginale의 抗原性에 관한 硏究

Study on the antigenicity of anaplasma marginale parasite in Korean native cattle

A splenectomized 5 month old Korean calf was inoculated with cryopreserved Anaplasma marginale infected blood. At peak parasitemia(40.1%) blood was collected, infected erythrocytes washed, lysed and A. marginale initial bodies isolated by differential centrifugation. Antigenic profile of isolated A. marginale organism was analized by SDS-PAGE and Western blotting technique. Coomassian blue stained SDS-PAGE gels revealed at least ten specific protein bands of approximately 28Kd-30Kd, 38Kd, 42Kd, 58Kd, 66Kd, 72Kd and 198Kd molecular weight. Only few protein bands recognized with polypeptides in the noninfected bovine erythrocyte antigen used as control. A. marginale protein recognized by specific anti-A. marginale antibodies demonstrated 8 polypeptide at 18KD, 28-31Kd, 38Kd, 55-58Kd, 97Kd, and 198Kd molecular weight range. Negative reactivities were observed with normal bovine serum with either A. marginale or erythrocytic antigens, respectively. Predominant protein were found at 18Kd, 38Kd and 97Kd molecular weight. On going studies will analyze the relative importance of those antigens.