전주근교 농장의 자돈에서 면역형광항체법을 이용한 돼지콜레라 혈청 중화항체가 조사

Survey of serum neutralizing antibody titers against hog cholera virus of piglet by the immunofluorescent method in the Chonju area

In order to evaluate the vaccination effect for hog cholera virus(HCV), sera were collected from each 20 piglets out of 26~32 days old before vaccination, and then from the 14th and 28th day post vaccination(PV) with LOM live virus vaccine at the J and C swine ranches in the suburbs of Chonju. Monoclonal antibody(MAb) against ALD strain of HCV and goat anti mouse FITC conjugates were employed in indirect immunofluorescence(IF) test for the titration of HCV and neutralization IF test for serum-neutralization(SN) titers against HCV. SN titers(M±S.D) were ＜2±0 in all piglets before vaccination at the both swine ranches. Whereas those of the J and C swine ranches were 111.2±15.08 and 144±121.7 at the 14th day PV and were 361.6±279.6 and 723.2±966.2 at the 28th day PV, respectively. Compared with Duncan’s test, SN titers were not significantly difference between the J and C swine ranches of the 14th day PV, but were significantly difference at the 28th day PV(P＜0.0009). Therefore, SN titers were significantly difference between the 14th and 28th day at the J or C ranches, respectively(P＜0.0007, P＜0.0114). Excepting SN titer was 1:16 in one piglet of the 14th day PV at the J swine ranch, defensible levels of SN titers could be detected in all piglets PV of the both swine ranches. It is suggested that varied effect of vaccination against HCV may be depended by feed and management, environment, sanitary conditions, breed and pedigree or vaccination program, etc.