겨울철 해양환경에서 vibrio parahaemolyticus의 생존조사

Survival of vibrio parahaemolyticus in marine environmentals during winter season

To study the survival of Vibrio parahaemolyticus in marine environmentals during winter season, V. parahaemolyticus were investigated from environmental sources in lower creek of 0-Sip chun once a month during June 1989 to May 1990. The results obtained were as follow: Water temperature were ranged 12℃ to 27℃ during a year at all stations observed, it were higher than 24℃ in August but lower than 12℃ in January at every stations. Concentration of sodium chloride In water samples were shown the highest level at A station (27.1-31.2%), 7 followed B(1.2-23.0%), C(1.1-9.7%), D(0.08-3.6%). And it was almost none of sodium chloride at E station. The pH values of water samples was ranged pH 6.7 to pH 8.4. It was stable at A station, while those in B, C and D station fluctuated irregularly. V. parahaemolyticus was isolated for the longest period at station C(from March to December), and followed at station B (from March to November). But V. alginolyticus was isolated at station C in January and February when V. parahaemolyticus was not detected. V. parahaemolyticus was detected longer period in mud than water at station C and D. V. parahaemolyticus was survived much longer period in mud (75 days) than in water (35 days)of station D at 12℃, also the bacteria was survived much longer period in 0.5% peptone water with 0.5% sodium chloride (for 9 days) than in 0.5% sodium chloride solution (for 1 day) at 12℃. Eighteen strains among 60 isolates showed positive reaction in Kanagawa-phenomenon test. After 42 negative strains were subcultured 5 times on Wagatsuma media, 31 strains (73.8%) among them produced Kanagawa hemolysin.