가축 유래 staphylococcus aureus의 enterotoxin 산생과 plasmid profile에 관한 연구 III. Coagulase, hemolysin과 enterotoxin type과의 관계 및 enterotoxin C.D. 균주의 독소산생성

Enterotoxin production and plasmid profiles of staphylococcus aureus isolated from domestic animals III. Correlation of Enterotoxin type with Coagulase type and Hemolysin Type, and Enterotoxin Production by Enterotoxin C and D Isolates

This study was conducted to investigate the correlation between coagulase, hemolysin and enterotoxin types of the S. aureus isolated from the domestic animals, and also examined were enterotoxin production of staphylococcal enterotoxin C and D strains isolated. No correlation was recognized between coagulase and enterotoxin types of the isolates but enterotoxin were produced by 68.1% of 276 coagulase positive S. aureus strains. In hemolytic reaction, all strains of S. aureus isolates from cattle and chickens were α-hemolytic while 67.8%, 17.9% and 14.3% of the isolates of swine origin were respectively α β-, α- and β-hemolytic. Hemolytic types were variable in the strains of men and bulk milk origin, and o-hemolytic strains were predominant in these samples. Though there was no correlation between hemolysin and enterotoxin types of the isloates, 74.3% of the organism produced one or more enterotoxin types. Generation time of type C strains were 23.38 and 24.24 minutes and were more rapid than those (24.24 and 25.90 minutes) of type D strains. The amount of enterotoxin produced by SEC strains was ≥8－≥10ng/m1 at 8 hours and in creased up to the miximum, ≥1,000ng/ml at 16 hours after incubation. Meanwhile, SED strains produced ≥4－≥8ng/ml of enterotoxin at 8 hours and the amount increased as little as ≥100ng/ml at 16 hours when compared to SEC strains. Maximum amount of the enterotoxin by SED strains was ≥200ng/ml at 24 hours after incubation.