조직중의 잔류 diethylstilbestrol과 zeranol의 동시분석법에 관한 연구

A study of residual diethylatibetrol and zeranol determinationin in tissues

A simultaneous analysis method using GC/MS was developed to monitor residual DES and zeranol. The residues in tissues were determined by the method developed. The residues in tissues were homogenized with sodium acetate buffer, hydrolysed with β-glucuronidase and extracted with acetonitrile. Lipids in the extract were removed with dichloromethane, the solvent was evaporated and then the residues were redissolved in alcohol mixture. Sep-pak cartridge was applied for clean-up and the separated residues were derivatized using TMSI and BSTFA. The residues were determined by GC/MS with DB-5 column, helium as a carrier gas, electron impact ionization mode and TIC and SIM scan mode. The limit of detection was 1 ppb and the calibration curves showed good linearity.