돼지 다발성장막염 및 관절염(glässer씨병)의 원인균 조사 및 분리균의 병발성에 관한 연구

Bacteriological studies on polyserositis and polyarthritis(glässer disease), and pathogenesis of the isolated organisms

Investigation into the bacteriology and epidemiology features of polyserositis and polyarthritis(Glässer disease) were carried out at 13 intensive pig farms of Chungnam and Kyounggi areas. Also determined were isolated frequency of hemophilus-like organisms in various sources of pigs, antibiotics susceptibilities of the isolates and pathogenesis of guinea pigs experimentally infected with isolates. The results obtained were summarized as follows: 1. Out of 75 hemophilus-like organisms isolated from 13 pig farms, 13 isolates of them were showed in same biological properties with standard strains. 2. Hemophilus-like organisms isolated from various samples of pigs belonged to 53 of 238 nasal discharges(22,3% ), 9 of 97 lungs(9.3%), 8 of 79 thoracic fluids(10.1%) and 1 of 22 others(4.6%), respectively. Urease-negative strains isolated from 6 of nasal discharge and 7 of lung (plus 5 strains from Bayer). 3. In regional distribution of 85 hemophilus-like organisms, the isolating rates of Pyeongtaek(26.3%) and Gongju(25%) were higher than that of Cheonan(17.5%), Hongseong(5.7%) and Asan(5%). Urease negative strains isolated from piggeries of Pyeongtaek(5), Asan(2) and Gongju(1) areas. 4. Among 13 farms, 9 farms were affected within the limits of 4.1%-47.2%, which the rates was decreased in good managing status, while increased in poor status or in respiratory symptoms. 5. The prevalence of isolates in four weeks of age pigs(31%) were the highest, in five weeks(22.5%) to in eight weeks(22.2%) to next higher ranks but lower in four weeks less(9.3%) and in eight weeks above(8.9%). 6. All isolates were highly susceptible to chloramphenicol, cephalothin, ampicillin, although they were highly resistant to streptomycin, neomycin, kanamycin. The drug susceptible properties against strains isolated from Japanese and Korean were showed in similar results. 7. The similar results of the pathological symptoms, bacteriological recovery and anatomic lesions after injection experimentally in guinea pigs with two urease-negative isolates and one Japanese strain(HPS) were discussed. The gross pathological finding of pig cases occurred naturally in pig farms was finally observed.