Enramycin 單一 및 複合抗生物質製劑의 檢定法 改善

Improvement of assay method for enramycin products

In Korea the use of Enramycin has begun since 1981 and the amount of use has increased due to its efficacy against gram-positive bacteria and little absorption into the gastro intestinal organ. At now Enramycin potency is being assayed by microbiological method like this : extraction solvent ; 2% HCI in MeOH or acetone : 4N HCI : D.W (40 : 15 : 45) and bacteria for assay ; Bacillus subtillus ATCC 6633. But this method is not suitable for products as treated with specially processed raw matarial or contaminated with lipid or protein which is a sort of media for cultivating enramycin producing bacteria. So improved method is needed and researched as follow. Such a condition as 1N HCI : acetone : D.W : 0.01% EDTA(4.3 : 29.7 : 51 : 15) shows best recovery rate. For assaying colistin potency in enramycin-colistin complex products such conditions as pH 6.0, 10% phosphate buffer or acetone : 1N HCI : pH 6.0, 10% phosphate buffer(5 : 0.5 : 94.5) show best recovery rate instead of pH 6.0, 1% phosphate buffer.