닭 coccidia의 純粹分離와 eimeria tenella의 鷄胎兒 培養에 관한 연구

Studies on purification of coccidia and cultivation of Eimeria tenella in chicken embryo

Sporozoites inoculated into the cecal of 3 weeks SPF chicken through the cloaca for the propargation and purification of Eimeria tenella and 5×105.8 oocyst was recovered. The population of oocyst and mortality rate of chicken embryo ating were affected by culture condition of Eimeria tenella. Maximum oocysts yields were obtained inoculated with 10³ sporozoite in 10 days chicken embryo on 8th day after inoculatin and yield of ocysts was 1×105.3±0.2. Nine to ten days chicken embryos suitable to culture host of E. tenella because vigorous cell differentiation of CAM observed at 9 days after incubation. The allantoic cavity was proper route of inoculation for the oocyst production and the numbers of oocyst per chicken embryo were 1105.3. Cell division multiplication observed in inner-cell membrane of CAM after sporozoite inoculation, and lst, 2nd and 3rd generation schizont produce a large quantity of merozoite at 48 hours, 72 hours and 144 hours after inoculation, respectively. Macrogametocyte differentiation appeared by the 168 hours and sufficient oocyst obtained after 7 to 10 days of inoculation. Maximum yield of oocyst recovered on the 8th day after inoculation that was 1×105.3 and significantly higher than the 7th day. Most of oocysts observed in the urate deposits of CAM and very few oocysts appeared in the allantoic fluid. The sporulation rate of harvested oocysts on 7th and 8th day after inoculation showed 0.1% and 31.6%, respectively. It was conclude that the high temperature culture(41℃) decrease the fertilization rate as time goes by. Inoculation of Sporozoite cause coccidiosis with haemorrhage and an inoculum of 10³, 10⁴ and 105 sporozoites killed 12.5%, 79% and 100% of of eggs from coccidiosis on 7th days after inoculation.