마우스에서 listeria monocytogenes의 장관정착과 그 결정요인 III. 장관정착에 미치는 요인시험

Intestinal colonization and its determinants by listeria monocytogenes in mice III. The factors related with intestinal colonization in mice

In SPF-ICR mice inoculated orally with Listeria monocytogenes investigated were effect of gastric juice, neutralizer of gastric acid, hemolysin and p60 for bacterial colonization. In in vitro stomach contents of heat-treated, HCI-treated to pH＜3.0, and normal contents as control, bacterial counts in heat-treated samples were similar to those in control group 8 hrs after inoculation. Bacterial growth was inhibited markedly In HCI-treated samples at 2 hrs after inoculation. Bacterial counts respectively of 3.16 log CFU /g and 2.66 log CFU /g in stomach of mice treated with sodium bicarbonate and ranitidine-HCI were prominent than those in control group(1.0 log CFU /g) at 2 hrs after inoculation. Moreover, the cecal colonization levels in sodium bicarbonate-treated group(5.57 log CFU /g) or ranitidine-HCI-treated group(5.13 log CFU /g) were higher than those of control group(2.27 log CFU /g). The cecal colonization level by L. monocytogenes strain Mackaness harvouring virulent factors as hemolysin and p60 was higher than those by such strains deficient for virulent factors as RI (hemolysin⁺p60⁻) or M3(hemolysin⁻ p60⁺).