학술저널
Affinity Chromatography법을 이용한 Protein G-HRP 효소접합체의 정제
Purification of Protein G-HRP Conjugate by Affinity Chromatography
- 한국예방수의학회
- Korean Journal of Veterinary Public Health
- Vol.19, No.4
-
1995.11337 - 341 (5 pages)
- 31
An immuno-affinity chromatography method was applied for the purification of protein G-Horseradish peroxidase emzyme conjugate (Prot G-HRP). The residual free HRP and free protein G were eliminated from Prot G-HRP conjugate solution by mouse IgG-Sepharose gel and chick anti-HRP-Sepharose gel respectively. When the affinity purified Prot G-HRP was used as the tracer of ELISA, it made the system improve in sensitivity.
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