Listeria spp. 표면단백질의 분석 및 면역원성

Immunogenicity and Analysis of Surface Protein of Listeria spp

This study is conducted to investigate specific surface proteins (SP) of Listeria spp. with SDS-PAGE and immunoblotting, and irnmunogenicity of specific SP in mice and Korean native goat immunized with those of Listeria monocytogenes. Profiles of L. monocytogenes were different from those of other species of Listeria. Surface protein with molecular weights of 62 and 68kd were usually found in all L. monocytogenes serovars. Strains of sv. 4b were characterized by the association of two major characteristic bands at 76 and 78kd, and sv 1/2 were characterized by the association of two major bands at 60 and 97kd. In spite of the weak similarity in SDS patterns, irnmunoblotting showed a large antigenic community among serovars or species: the 4b antiserum reacted in the profiles of all Listeria spp. with two major band at 76 and 78kd, and the 1/2b antiserum reacted in the profiles of all Listeria spp. with two major band at 68 and 76kd. Antigenic titers of specific SP in mice and in Korean native goat were 5,120 for 78kd, 2,560 for 62kd(in goat, 1,280), and 640 for 97kd.