Eacherichie coli O157: H7 으로부터 분리한 Bacteriophages의 특성

Characteristics of Bacteriophages Isolated from Eacherichie coli O157:H7

Bacteriophage encoding toxin gene Was lysogenized from Escherichia coli O157:H7 by the method of UV irradiation. This phage was examined its morphology by electronmicroscopy and was investigated its stability to temperature, pH stability, inactivation by UV irradiation and characteristics of phage DNA. The morphology of bacteriophage lysogenized from verotoxin-2-producing Escherichia coli had head and tail as typical bacteriophage, The phage was stable in wide pH range (pH 6~10), and optimal pH for survival was pH 7.0. In temperature stability, phage was most stable at 35℃ and mostly inactivated by UV irradiation for 100 sec. Adsorption rates of the phage on lawn cell (E.coli C600) was represented beyond 90% by mixed incubation for 35 min.