Verocytotoxin 생성 대장균의 분포 및 Receptor를 이용한 RELISA 법의 개발 I. 국내 가축에 대한 생성 대장균의 분포조사 및 Hybrid Toxin의 조합

Distribution of Verocytotoxin-Producing Escherichia coli and Development of Receptor Binding Enzyme-Linked Immnuosorbent Assay I. Combination of Hybrid Toxins and Distribution of Verocytotoxin-Producing Escherichia coli against Domestic Animal in Korea

Verocytotoxin-producing Escherichia coli(VTEC) as etiological agents of diarrhea represents one of the most recent observations in the field of enteric infections. In epidemiological survey related to VTEC infection of cattle and pigs in Korea, a total of 675 stools of cattle and 784 stools of pigs collected at slaughterhouse were examined for the presence of VTEC. The isolation rates of VTEC from cattle and pigs were 14.4% and 11.4%, respectively. Particularly, 2 strains of E. coli O157:H7 were isolated from cattle. Cytotoxicity for culture supernatants of isolates was assayed to Vero and HeLa cell monolayers. The level of cytotoxin produced by isolated strains were 102~107 cytotoxic dose (CD50)/ml of incubated supernatants.