Ochratoxin A가 신장근위세뇨관 상피세포의 유기물질 운반에 미치는 영향

Effects of ochratoxin A on the transport of organic substances in basolateral and brush border membrane vesicles from the rabbit kidney cortex

Basolateral and brush border membrane vesicles (BLMV and BBMV) of renal proximal tubules were prepared from adult male New Zealand White rabbits to evaluate as a potential model for the assessment of ochratoxin A (OTA)-induced nephrotoxicity. PAH uptkes using BLMV, glucose and leucine uptakes using BBMV were measured in the rabbits treated with OTA. In addition, urinalysis and histopathological studies were performed to investigate the correlationship with membrane vesicle uptakes. In the uptake experiments, OTA decreased PAH uptakes in BLMV, glucose and caused cellular sweling and congestion of renal proximal and distal tubules. Taken together, results obtained by renal membrane vesicle uptake system corresponded weil with urinalysis and histopathologic findings, and suggest that this uptake system may be valuable for evaluating the nephrotoxicity of toxicants including OTA.