활성을 띤 활성산소들의 반응에 대한 멜라토닌의 영향

Effect of melatonin on oxidative reactions by reactive oxygen species

과산화 음이온, 과산화수소 및 hydroxyl rad-ical의 반응에 대한 멜라토닌의 효과를 화학적으로 증명하는 in vitro 시험을 실시하여 다음과 같은 결론을 얻었다. 멜라토닌은 과산화 음이온에 의한 ferricytochrome c의 환원을 억제하지 못하였으며 따라서 과산화 음이온 제거 능력이 없었다. 멜라토닌은 과산화수소에 의한 ferrocytochrome c의 산화를 강력하게 억제하였으며 특히 0.5mM 이하의 농도에서 80% 이상 억제하였다. 멜라토닌은 hydroxyl radical에 의하여 deoxyribose로부터 thiobarbituric acid-reactive substance의 생성을 억제하였으며 특히 0.3mM이하의 저 농도에서 높은 산화 억제 효율을 나타내었으며 0.6mM이상의 농도에서 효율이 낮았다.

In order to investigate the direct effect of melatonin to protect against the oxidative reactions induced by superoxide, hydrogen peroxide and hydroxyl radical changes of absorption spectrum of cytochrome c in the presence of superoxide or hydrogen peroxide, and the oxidation of deoxyribose in hydroxyl radical generating system in vitro were examined respectively. No inhibition of superoxide-dependent reduction of ferricytochrome c by melatonin was observed. Melatonin inhibited the oxidation of ferrocytochrome c by hydrogen peroxide almost entirely (80%) with lower concentration than 0.5mM melatonin Melatonin efficiently inhibited formation of thiobarbituric acid-reactive substances from deoxyribose oxidation by hydroxyl radicals but the higher concentrations than 0.6mM melatonin were not so efficient as lower concentration.