가축사육장 및 작업장 폐수로부터 listeria monocytogenes의 분리 및 분리균의 약제 내성

Prevalence and antibiotic-resistance of listeria monocytogenes from sewage of livestock farms and product processing plants

리스테리아병에 대한 역학적 연구의 일환으로 가축사육장, 우유처리장, 도축장 및 도계처리장에서 유출되는 폐수로부터 L. monocytogenes를 분리하고, 혈청형의 분포와 항생물질에 대한 감수성 태도를 조사하였다. L. monocytogenes 분리율은 도축장 폐수의 31.8%, 도계장 폐수의 11.7% 및 유처리장 폐수의 5.0%이었고, 우사, 돈사 및 계사 폐수에서는 분리되지 않았다. 분리균의 혈청형은 총 193주 중 serotype 1이 82.4%, serotype 4가 17.6%로 분류되었고, 유가공장 폐수에서는 serotype 4가, 도축장 및 도계장 폐수는 serotype 1이 대부분을 점하였다. 분리균의 항생물질에 대한 내성율은 colistin(Cl)에 100%, nalidixic acid(Na)에 96.0%, tetracycline(Tc)에 46.0%, norfloxacin 1.6%이었고, ampicillin, cephalothin, chloramphenicol, penicillin 및 vancomycin에는 100% 감수성을 나타내었다. 그리고 약제 내성 유형은 ClㆍNa유형(48.7%)과 ClㆍNaㆍTc유형(45.6%)이 대부분이었다.

To investigate the epidemiological trait of listeriosis, Listeria monocytogenes were isolated from sewage of livestock farms, milk plants, slaughterhouses and poultry processing plants in Gyeongnam during the period from June 1998 to March 1999. Total 193 strains isolated were examined for serotypes and antibiotic susceptibility. L. monocytogenes were isolated from 31.8% of slaughterhouse sewage, 11.7% of poultry processing plant sewage and 5.0% of milk plant sewage but was not found in the sewage of cattle pen, pigpen and poultry houses. The serovars of 193 isolates from sewage of product processing plants were serotype 1(82.4%) and serotype 4(17.6%). The majority of the isolates from milk plant sewage were serotype 4, and those from slaughterhouse sewage were serotype 1. Antibiotic susceptibility test of all Listeria strains isolated were performed by disk agar diffusion method, using 9 antibiotics as follows; ampicillin(Am), cephalothin(Ct), chloramphenicol(Cm), colistin(Cl), nalidixic acid(Na), norfloxacin(Nf), penicillin(Pc), tetracycline(Tc) and vancomycin(Vm), Of the 193 Listria isolates, 100% were resistant to Cl, 96.0% to Na, 46.0% to Tc and 1.6% to Nf. However, all isolates were 100% sensitivity to antisiotics such as Am, Ct, Cm, Pc and Vm. The drug resistance patterns of 193 resistant strains were distributed from one to three fold pattern, and the most common resistant patterns were ClㆍNa(48.7%) and ClㆍNaㆍTc(45.6%).