PCR기법을 이용한 돼지 적리균의 신속 검출

Rapid detection of serpulina hyodysenteriae by polymerase chain reaction

A polymerase chain reaction (PCR) for detection of Serpulina hyodysenteriae, the etiological agent of swine dysentery, was studied. The primers for specific detection of S. hyodysenteriae was developed from the part of the DNA sequences obtained from a 1,700 kilobase diagnostic probe specific for S. hyodysenteriae. Sequence information from approximately 320 bp of the probe, referred to as the BGC sequences was available for use in PCR. These primers were expected to amplify a product of approximately 180 bp. On the banding patterns by electrophoresis, this amplified product was verified. For the evaluation of the specificity of the primer, PCR reaction was tested on purified DNA from strains of S. hyodysenteriae and, strains of other related intestinal spirochaetes. As a result, these primers were shown to be specific for all S. hyodysenteriae. Also these primers could be used to detect as little as from 1 pg to 100 pg DNA purified from S. hyodysenteriae. Therefore, it was considered to be a basic data to develope the diagnostic tools of specific, sensitive and rapid detection for S. hyodysenteriae in feces.