Human dermal stem/progenitor cell-derived conditioned medium enhance moisturizing of normal human epidermal keratinocytes

This study was performed to define the epidermal moisturizing effects of human dermal stem/progenitor cell-derived conditioned medium (hDSPC-CM) on normal human epidermal keratinocytes (NHEKs). To investigate epidermal moisturizing effect of hDSPC-CM on NHEKs, I measured superoxide scavenging activity, cell viability assay, gene expressions, and hyaluronic acid (HA)-ELISA assay. In this study, I could elucidate the effects of hDSPC-CM on AQP3, HAS2, KRT1, and KRT10 mRNA expressions and hyaluronic acid production. Quantitative Real-time RT-PCR presented that hDSPC-CM increased gene expression levels of HAS2, AQP3, KRT1, and KRT10 and HA-ELISA also revealed that hDSPC-CM increased HA production in NHEKs. I identified the epidermal moisturizing effects of hDSPC-CM, and this outpot showed that the hDSPC-CM can be a potent moisturizing cosmetic ingredient for epdiermal moisturizing. Based on this study, I anticipated further studies about the mechanisms of hDSPC-CM on epidermal keratinocytes to develop not only cosmetics but for healthcare medicines.