시금치 에탄올추출물의 뷰티푸드 소재로서의 활용 가능성 연구

The Utilization of Spinach Ethanol Extract as a Beauty Food Material

This study, investigated the amounts of lutein and total polyphenols, anti-oxidative effects, antimicrobial and the anti-inflammatory activities of spinach ethanol extracts to evaluate their use as functional ingredients for skin health and beauty food. The amounts lutein and total polyphenols were 131.8 ㎎/100g dry weight(dw) and 116.39±0.66 ㎎/g in spinach ethanol extract, respectively. In the antioxidant assay, the electron donating ability and ABTS radical scavenging activity were significantly increased in a concentration-dependent manner and the antioxidant effect were 47.28% and 97.49% at a concentration of 5,000 ㎍/㎖, respectively. The antimicrobial activities against S. aureus, S. epidermidis, E. coli and P. acnes showed a high inhibition of growth of 13.3∼18.0 mm. Especially, the antimicrobial activity against P. acnes, an inflammatory acne bacterium, was significantly higher. The cytotoxic concentrations of spinach ethanol extracts showed cell viability above 100% at concentrations 1,000 ㎍/㎖ and inhibition of NO production by 57.3% at 1,000 ㎍/㎖. Also the expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) proteins were significantly inhibited in a concentration-dependent manner and showed inhibition rates of 45.1% and 39.4% at 1,000 ㎍/㎖. Accordingly, it can be concluded that spinach ethanol extracts have the potential to be used as a functional ingredient for skin health and beauty food.