황산카드뮴독성의 산화적 손상에 대한 부들 추출물의 항산화 효과

Antioxidative Effect of Typha orientalis L. Extract on the Oxidative Stress Induced by Cytotoxicity of Cadmium Sulfate

Objectives: This study was carried out to analyze the cytotoxicity of cadmium sulfate (CdSO₄) and the antioxidative effect of Typha orientalis L. (TO) extract on the oxidative stress induced by cytotoxicity of CdSO₄ in the cultured NIH3T3 fibroblasts. Methods: For this study, the cell viability and the antioxidative effects such as the inhibitory activity of lipid peroxidation (LP) and superoxide dismutase (SOD)-like activity and xanthine oxidase (XO)-inhibitory activity were assessed. Results: The cadmium sulfate significantly decreased cell viability in dose-dependently, and XTT50 value was measured at 47.4 µM of CdSO₄. The cytotoxicity of CdSO₄ was determined as highly toxic by Borenfreund and Puerner s toxic criteria. The butylated hydroxytoluene (BHT) as antioxidant significantly increased cell viability injured by CdSO₄-induced cytotoxicity in these cultures. In the protective effect of TO extract on CdSO₄-induced cytotoxicity, TO extract remarkably increased the inhibitory ability of LP and XO as well as SOD-like ability. Conclusions: From the above results, it is suggested that the oxidative stress is involved in the cytotoxicity of CdSO₄, and TO extract effectively protected CdSO₄-induced cytotoxicity by antioxidative effects. The natural component like TO extract may be a putative therapeutic agent for treatment of the toxicity induced by heavy metallic compound like CdSO₄ correlated with the oxidative stress.