Objectives: This study was carried out to analyze the cytotoxicity of cadmium sulfate (CdSO<sub>4</sub>) and the antioxidative effect of Typha orientalis L. (TO) extract on the oxidative stress induced by cytotoxicity of CdSO<sub>4</sub> in the cultured NIH3T3 fibroblasts. Methods: For this study, the cell viability and the antioxidative effects such as the inhibitory activity of lipid peroxidation (LP) and superoxide dismutase (SOD)-like activity and xanthine oxidase (XO)-inhibitory activity were assessed. Results: The cadmium sulfate significantly decreased cell viability in dose-dependently, and XTT50 value was measured at 47.4 µM of CdSO<sub>4</sub>. The cytotoxicity of CdSO<sub>4</sub> was determined as highly toxic by Borenfreund and Puerner s toxic criteria. The butylated hydroxytoluene (BHT) as antioxidant significantly increased cell viability injured by CdSO<sub>4</sub>-induced cytotoxicity in these cultures. In the protective effect of TO extract on CdSO<sub>4</sub>-induced cytotoxicity, TO extract remarkably increased the inhibitory ability of LP and XO as well as SOD-like ability. Conclusions: From the above results, it is suggested that the oxidative stress is involved in the cytotoxicity of CdSO<sub>4</sub>, and TO extract effectively protected CdSO<sub>4</sub>-induced cytotoxicity by antioxidative effects. The natural component like TO extract may be a putative therapeutic agent for treatment of the toxicity induced by heavy metallic compound like CdSO<sub>4</sub> correlated with the oxidative stress.
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II. 연구 방법
III. 결 과
IV. 고 찰
V. 결 론