
The Ultrastructure of Corneal Epithelium after Co-cultivation with Herpes Simplex Virus
- Dong Yeon Lee Myung Kyoo Ko Joon Kiu Choe
- 대한안과학회
- The Korean Journal of Ophthalmology
- Vol.12 No.2
- 등재여부 : KCI등재
- 1998.12
- 103 - 107 (5 pages)
To elucidate the ultrastructural change of corneal epithelium co-cultured with herpes simplex virus (HSV), the corneal epithelium of 3 rabbits was excised and cultivated in culture media. After 7 days, the Kos strain of herpes simplex virus was inoculated in the cultured cornea epithelium until cytopathic effect was occurred. It was fixed in the solution of 3% glutaraldehyde and examined with electronmicroscope. In co-cultured cells, the intercellular spaces had increased and microvilli were seen prominently. The virus particles that initiated the infection by fusing the viral envelope with the plasma membrane were also seen. The nuclear degeneration in an infected cell was prominent. The nuclear membrane was folded markedly, and the chromatin was degraded, condensed and displaced toward the nuclear membrane.
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES