Alloxan에 의해 유발된 산화스트레스에 대한 과일과 채소 추출물의 췌장베타세포 생존율과 항산화 효소활성 효과

Screening of Ethanol Extracts of Korean Fruits and Vegetables for Cell Viability and Antioxidant Enzyme Activity on Alloxan-Induced Oxidative Stress in Pancreatic Beta Cell

The present study was taken to screen the anti-oxidative effects of several fruits and vegetable ethanol extracts(FVE) against alloxan(AXN)-induced oxidative stress in pancreatic β-cells, HIT-T 15. To evaluate the cytoprotective potential effect of FVE, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide(MTT) cell proliferation assay, lactate dehydrogenase(LDH) release assay, NAD⁺/NADH ratio, insulin secretion and antioxidant enzyme activity(SOD, GST, GR and GPx) were assessed. All of those extracts significantly increased cell viability which was decreased by AXN. Of these 13 extracts, 11 extracts(Allium sativum, Petasites japonicus and Schisandra chinensis, etc.) were significantly lowered LDH leakage and 9 extracts(Allium sativum, Prunus salicina and Youngia sonchifolia, etc.) were significantly elevated NAD⁺/NADH ratio. The insulin secretion was also increased by extracts such as, Allium sativum, Cornus officinalis and Youngia sonchifolia. Moreover, 5 extracts, including Perilla frutescens, Prunus persica for. alba, Prunus persica for. rubro-plena, Prunus salicina and Schisandra chinensis, showed significantly increased antioxidant enzyme activities compared with AXN-treated cells. From these findings, it is suggested that stem of Allium sativum, fruit of Cornus officinalis, fruit of Prunus salicina, fruit of Schisandra chinensis and aerial part of Youngia sonchifolia have significantly potential protective effects against AXN-induced oxidative stress damage in pancreatic β-cells. We therefore suggest the screened fruits and vegetables could be a promising source as the effective and safe ingredients for developing functional foods.