명아주 추출물의 항산화 효과에 의한 배양 인체피부섬유모세포에 대한 카드뮴 손상방어

Protection of Chenopodium album var. centrorubrum Extract by Oxidative Effect in Cultured Human Skin Fibroblasts Damaged by Cadmium

To evaluate the cytotoxicity of cadmium(Cd), an environmental pollutant, and the protective effect of Chenopodium album var. centrorubrum(CAC) extract on the Cd-induced cytotoxicity in cultured human skin fibroblasts(Detroit 551). Cultured Detroit 551 cells were treated or pretreated with cadmiun chloride(CdCl₂) or CAC extract. In this study, CdCl₂ significantly decreased cell viability compared with control, and XTT₅₀ or XTT₅₀ value(midcytotoxicity value, MCV) was determined at a concentration of 41.1μM or 43.1μM. The MCV of CdCl₂ was highly toxic by the criteria of Borenfreund and Puerner. While, CdCl₂-induced cytotoxicity was prevented by superoxide dismutase(SOD), an antioxidative enzyme. In the protective effect of CAC extract on CdCl₂-induced cytotoxicity, CAC extract prevented CdCl₂-induced cytotoxicity by the significant increase of cell viability decreased by CdCl₂-induced cytotoxicity. And also, CAC extract showed antioxidative effects such as electron donating activity(EDA) and inhibitory activity of lipid peroxidation(LP). From above the results, it is suggested that CdCl₂ was highly toxic to cultured Detroit 551 cells and CAC extract was effective in the blocking CdCl₂-induced cytotoxicity by the antioxidative effects such as EDA and inhibitory activity of LP. In conclusion, the physical activity of natural component such as CAC extract may be a useful resource for preventing diseases mediated by reactive oxygen species(ROS) such as heavy metal toxication, stroke or dementia.