배양 골모세포에 있어서 골다공증의 유발물질인 카드뮴에 대한 우슬 추출물의 보호 효과

Protective Effect of Achyranthis radixs Extract on Cadmium of Osteoporosis-Induced Agent in Cultured Osteoblasts

To evaluate the cytotoxicity of osteoporosis-induced agent, cadmium (Cd) and the protective effect of Achyranthis radix (AR) extract on the Cd-induced cytotoxicity in cultured osteoblasts (HFOB-1). Cultured HFOB-1 cells were treated or pretreated with cadmiun chloride (CdCl₂) or AR extract. In this study, CdCl₂ significantly decreased cell viability compared with control, and XTT₅₀ value (midcytotoxicity value, MCV) was determined at a concentration of 42.0uM. The CdCl₂ was toxic by the significant decrease of cell viability. While, CdCl₂-induced cytotoxicity was prevented by superoxide dismutase (SOD) or butylated hydroxytoluene (BHT), the antioxidative agents. In the protective effect of AR extract on CdCl₂-induced cytotoxicity, AR extract prevented CdCl₂-induced cytotoxicity by the significant increase of cell viability decreased by CdCl₂-induced cytotoxicity. And also, AR extract showed antioxidative effects such as electron donating activity (EDA) and inhibitory effect of lipid peroxidation (LP). From these results, it is suggested that CdCl₂ showed toxic effect on cultured osteoblasts, and the cytotoxicity of CdCl₂ was involved in oxidative stress. And also, AR extract was effective in blocking CdCl₂-induced cytotoxicity by antioxidative effect. Conclusively, the natural components such as AR extract may be a useful resources for preventing diseases mediated by reactive oxygen species (ROS) such as stroke or ischemia.