배양 신경교종세포에서 치매유발물질인 알루미늄의 세포독성 및 쇠비름 추출물의 보호효과

Cytotoxicity and Protective Effect of Portulaca oleracea L. Extract on Cultured Neuroglioma Cells Damaged by Aluminum of Dementia-Induced Agent

To evaluate the cytotoxicity of dementia-induced agent, aluminum(Al) and the protective effect of Portulaca oleracea L.(PO) extract on the Al-induced cytotoxicity in cultured neuroglioma cells(C6 glioma). Cultured C6 glioma cells were treated with aluminum chloride(AlCl₃) or PO extract. In this study, AlCl₃ significantly decreased cell viability compared with control, and XTT₅₀ was determined at a concentration of 121.5uM. Therefore, AlCl₃ was midcytotoxic by the criteria of Borenfreund and Puerner. While, AlCl₃-induced cytotoxicity was prevented by vitamin E, an antioxidative enzyme. In the protective effect of PO extract on AlCl₃-induced cytotoxicity, PO extract prevented AlCl₃-induced cytotoxicity by the significant increase of cell viability. And also, PO extract showed antioxidative effects such as superoxide dismutase(SOD)-like activity, the inhibitory activity of lipid peroxidation(LP) and the decrease of lactate dehydrogenase(LDH) activity. it is suggested that AlCl₃ was midcytotoxic to cultured C6 glioma cells and the cytotoxicity was involved in oxidative stress. PO extract was effective in the prevention of AlCl₃-induced cytotoxicity by the antioxidative effect. Therefore, natural component such as PO extract may be a putative resources for preventing reactive oxygen species(ROS)-related diseases such as dementia or stroke.