배양 흑색종세포에 있어서 NiSO₄의 세포독성 및 멜라닌화에 대한 감잎 추출물의 영향

Effect of Persimmon Leaves Extract on the Cytotoxicity and Melanogenesis of NiSO₄ in Cultured Melanoma Cells

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To evaluate the effect of persimmon(Diospyros kaki Thunb.) leaves(PL) extract on the cytotoxicity and melanogenesis of nickel sulfate(NiSO₄), an environmental pollutant or an allergic contact dermatitis inducer, the colorimetric assay was performed to measure the cell viability and lactate dehydrogenase(LDH) activity as well as the tyrosinase activity and total amount of melanin in cultured B16/F10 melanoma cells. In this study, NiSO₄ significantly decreased cell viability in dose-dependent manner, and XTT₅₀ value was calculated at 106.7μM of NiSO₄. The cytotoxicity of NiSO₄ was revealed to be mid-toxic in these cultures by Borenfreund and Puerner’s toxic criteria. And also, vitamin E effectively prevented NiSO₄-induced cytotoxicity by the significant increase of cell viability. PL extract showed the antioxidative activity by the significant increase of cell viability and the decrease of LDH activity compared to positive control(NiSO₄). In the melanogenesis, PL extract significantly decreased both tyrosinase activity and total amount of melanin which were increased by NiSO₄-induced cytotoxicity. From these findings, it is suggested that the cytotoxicity of NiSO₄ is involved in oxidative stress, and PL extract effectively prevented the cytotoxicity and the melanogenesis induced by NiSO₄. Conclusively, natural component such as PL extract may be a putative resources for beauty or detoxification by the prevention of the toxicity and the melanogenesis of heavy metals related with oxidative stress such as NiSO₄.