관중속 3종의 메탄올 추출물 및 참지네고사리 분획물의 α-glucosidase 억제 활성

Alpha-glucosidase Inhibition Activity of Methanol Extracts Obtained from Three Dryopteris Species and Fractions Obtained from D. nipponensis

This study was performed to analyze the α-glucosidase inhibitory activity in fronds of 3 Dryopteris species, D. crassirhizoma, D. nipponensis and D. fragrans var. remotiuscula, and in rhizomes of D. crassirhizoma and D. nipponensis, and then to explore the natural materials for developing natural α-glucosidase inhibitors. An analysis was performed using the crude extract of fronds and rhizomes obtained through 30 minutes ultra sonification extraction in 42 kHz ultrasonic water tank with 100% methanol, as a solvent, after the freeze drying and grinding. As a result of the analysis of α-glucosidase inhibitory activity, in respect of IC₅₀ values of fronds and rhizomes, D. nipponensis was the highest as 4.13 and 2.19μgㆍmL⁻¹ respectively among 3 Dryopteris species and their inhibitory activities were 342 and 646 times higher than acarbose(IC₅₀=1413.70μgㆍmL⁻¹) used as control group, respectively. In respect of the biomass required to inhibit 50% of α-glucosidase activity, the fronds and rhizomes of D. nipponensis required the least as 0.04 and 0.05mg, respectively. From the crude extract, D. nipponensis with high α-glucosidase activity and less required biomass was selected. And then the crude extract was subdivided by the solution in the order of n-hexane, chloroform, ethyl acetate, n-butanol and water in order to analyze α-glucosidase inhibitory activity as well as the biomass required for each fraction comparatively. The fronds of D. nipponensis was high in n-butanol fraction(1.09μgㆍmL⁻¹), and the rhizome was high in water fraction(9.36μgㆍmL⁻¹), indicating the inhibitory activity 1297 and 151 times higher than acarbose, respectively. The required biomass of the fronds and rhizomes of D. nipponensis were highest in n-butanol and water fraction. As a result of this study, the crude extract of D. nipponensis showed high α-glucosidase inhibitory activity requiring a small amount of biomass. Thus, its value for the development to antidiabetic agent is considered very high.