발아콩에서 대사유도물질 처리에 의한 isoflavone 대사 관련 유전자 IFS와 IFR의 발현 양상과 isoflavone 생합성 변화

Manipuation of Isoflavone Levels in Sobyean Sprouts and Expression Pattern of Two Genes Coded for Isoflavone Reductase and Isoflavone Synthase by Chemical Treatments

Isoflavone is synthesized in several different organs such as root, leaf and developing and germinating seed. Isoflavone content reflects overall history of physiological event given to a plant during certain period of time. Amount of isoflavone in a organ or tissues is controlled by two key enzymes of isoflavone metabolism, isoflavone synthase and isoflavone reductase, ultimately. Many genes engaged in isoflavone synthesis response to biotic and abiotic stresses. In our previous study, it appeared that isoflavone content in germinating mung bean seed is increased by stress related chemical compound, for example, salicylic acid. Here, we found that both genes encoding isoflavone synthase and isoflavone reductase are over-expressed in germinating soybean seed by salicylic and acetyl salicylic acid treatment. These over-expression of both genes led to enhenced level of isoflavone in germinating soybean seed.