Inter simple sequence repeat (ISSR) marker를 이용한 구기자의 유전적 유연관계 분석

Assessment of Genetic Relationship among Boxthorn (Lycium chinensis Mill.) Accessions by ISSR Markers

This study was conducted to assess genetic diversity among 16 genotypes of boxthorn (Lycium chinensis Mill.) using inter-simple sequence repeat (ISSR) markers. The 18 ISSR primers out of 100 primers showed the amplification of 101 reproducible fragments. A total of 56 DNA fragments were polymorphic with an average 3.1 polymorphic bands per primer. The polymorphic primers were divided into 16 anchored primers and 2 non-anchored primers. All of the anchored primers were di-nucleotide repeat motif, and polymorphism level was higher in the primers with poly GA and CT motif than CA and GT motif primers. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Sixteen boxthorn varieties and accessions were separated into 2 distinctive groups and genetic distance of cluster ranged from 0.82 to 0.97. Eighteen markers were able to distinguish every variety. Therefore, ISSR markers may be suitable for characterizing the large numbers of germplasms and fingerprinting of boxthorn varieties. This study was conducted to assess genetic diversity among 16 genotypes of boxthorn (Lycium chinensis Mill.) using inter-simple sequence repeat (ISSR) markers. The 18 ISSR primers out of 100 primers showed the amplification of 101 reproducible fragments. A total of 56 DNA fragments were polymorphic with an average 3.1 polymorphic bands per primer. The polymorphic primers were divided into 16 anchored primers and 2 non-anchored primers. All of the anchored primers were di-nucleotide repeat motif, and polymorphism level was higher in the primers with poly GA and CT motif than CA and GT motif primers. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Sixteen boxthorn varieties and accessions were separated into 2 distinctive groups and genetic distance of cluster ranged from 0.82 to 0.97. Eighteen markers were able to distinguish every variety. Therefore, ISSR markers may be suitable for characterizing the large numbers of germplasms and fingerprinting of boxthorn varieties.