CAX1 유전자가 도입에 의한 칼슘강화 벼 계통의 분석

Evaluation of Ca²⁺-enhanced Rice Plant Expressing the Arabidopsis Ca²⁺/H⁺ Antiporter CAX1 Gene

This study was carried out to analyze the mineral contents including calcium in brown rice for the rice plants transformed with CAX1 gene. In the previous study the transgenic rice plants overexpressing the Arabidopsis Ca²⁺/H⁺ antiporter CAX1 gene were developed using Agrobacterium-mediated transformation method. The calcium content in brown rice of the transgenic rice plants was ranged from 2.7 to 14 times higher than that of the donor cultivar. SEM (scanning electronic microscope) analysis showed that the starch structure of transgenic rice comprised round and tightly packed granules compared with the donor cultivar. The major agronomic traits such as culm length, panicle length and panicle number of transgenic plants (T₃) were similar to the donor cultivar. The grain shape of the T₃-17 and T₃-25 was different from the donor cultivar.