Comparative Proteome Analysis of Seed Storage Proteins in Allogamous and Autogamous Buckwheat

Seed proteins of allogamous buckwheat (Fagopyrum esculentum Moench. cv. Miyazakizairai) and autogamous buckwheat were separated by two-dimensional gel electrophoresis (2-DE) and characterized by gasphase sequencing. Total of 100 protein spots were resolved in 2-DE gels. Ten spots that were specific for autogamous buckwheat were characterized. These proteins could be used as markers for the identification of autogamous cultivar. Randomly selected 17 proteins were subjected to Nterminal sequencing. Nterminally blocked proteins (~80%) were subjected to Cleveland peptide mapping followed by microsequencing. The sequences obtained were compared with those of the SWISS-PROT and NCBI databases. A protein showed high sequence homology with 22 kDa allergen protein of buckwheat and seven with proteins such as allergen Ara H1, cysteine proteinase 2, ribosomal protein, superoxide dismutase from rice, Arabidopsis, peanut and maize, respectively. In autogamous buckwheat, the N-terminal sequence of three proteins showed homologous to glutelin type-A III precursor, glutelin type-B 2 precursor and putative 30S ribosomal protein S31 from rice.