유전자 재조합 대장균을 이용한 페닐알라닌 생산성 및 DAHP 합성효소의 활성도 검토

A Study on the Phenylalanine Productivity and DAHP Synthase Activity by Recombinant Gene in Escherichia coli

The production of aromatic amino acids were regulated by 3 initial regulatory enzymes thoses were 3-deoxy-D-arabinoheptulose-7-phosphate synthetases which codes by Phenylalanine, Tyrosine, Tryptophan and named aroG, aroF, aroH respectively. The plasmid, pSY160-14 including the aroG gene has newly constructed and is transferred to AT2471. DAHP synthetase activity with aroG gene in host cell was detected 22% higher compared to pSY130-14 at 37℃. As these results, it appears that the plasmid with aroG gene was considered directly regulated and inhibited to DAHP synthetase and also observed the Phenylalanine productivity and gene expression were detected 21%, 22% respectively better than control below 40℃.