Streptomyces sp. ᄂ79-290 배양액 유래의 산성지 용성 항균활성 물질의 분리정 제
Studies on Acidic lipophilic A ntibiotic from Culture Broth of Streptomyces sp. L79-290
- 한국산업기술융합학회(구. 산업기술교육훈련학회)
- 산업기술연구논문지
- 산업기술교육훈련논문지 제19권 3호
- : KCI등재후보
- 2014.09
- 79 - 85 (7 pages)
In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L79-290 was discovered which produced a water-nonsoluble acidic antibiotic L79-290. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L79-290 was tested by several carbon and nitrogen sources such as maltose and peptone, respectively. L79-290 showed anti-microbial activities against several Gram-positive and Gram-negative bacteria. From the culture supernatant, L79-290 was purified by silicagel column chromatography follow after solvent and water extraction. In the pH stability test, L79-290 showed good stability over 80% under acidic and alkali conditions, respectively. Based on the spectroscopic measurement including ultraviolet(UV), infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrom analyses, L79-290 was identified as novobiocin. Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic that is produced by Streptomyces niveus.
I . 서 론
I I . 실험 방법
III. 실험결과 및 고찰
IV. 결 론
참고문헌