Streptomyces sp. ᄂ79-290 배양액 유래의 산성지 용성 항균활성 물질의 분리정 제

Studies on Acidic lipophilic A ntibiotic from Culture Broth of Streptomyces sp. L79-290

In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L79-290 was discovered which produced a water-nonsoluble acidic antibiotic L79-290. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L79-290 was tested by several carbon and nitrogen sources such as maltose and peptone, respectively. L79-290 showed anti-microbial activities against several Gram-positive and Gram-negative bacteria. From the culture supernatant, L79-290 was purified by silicagel column chromatography follow after solvent and water extraction. In the pH stability test, L79-290 showed good stability over 80% under acidic and alkali conditions, respectively. Based on the spectroscopic measurement including ultraviolet(UV), infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrom analyses, L79-290 was identified as novobiocin. Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic that is produced by Streptomyces niveus.