CHO 세포에서 재조합 단백질의 생산에 대한 저온 배양의 영향

Effect of Low Culture Temperature orr Recombinant Protein Production in Chinese Hamster Ovary Cells

Mammalian cell culture is typically operated at the physiological temperature of 370C. However, recent research has shown that the use of low-temperature conditions results in changes in cell characteristics, such as increased specific productivity and extended periods of cell viability, that can potentially improve the production of recombinant proteins. Suspension culture of recombinant Chinese hamster ovary (CHO) cells producing recombinant anti-IL-8 antibody was performed to investigate the effect of anti-IL-8 antibody production at low culture temperature. In batch culture at 330C and 30°C, cell growth was suppressed, but cell viability remained high for a longer culture period. This enhanced anti-IL-8 antibody production was not just because of enhanced specific anti-IL-8 antibody productivity, but mainly because of the extended culture longevity. Taken together, this result demonstrates that the application of low culture temperature is feasible to enhance the production of anti-IL-8 antibody in rCHO cells.