재조합 단백질의 생산성에 대한 저온 배양의 영향 고찰

Impact of Mild Hypothermic Culture on the Productivity of Recombinant Protein

Mammalian cells producing recombinant proteins were typically cultured at the physiological temperature of 37℃. However, subphysiological culture of CHO cells suppressed cell growth, resulting in decrease of recombinant protein productivity in comparison to standard batch maintained at 37℃. At low temperature, cells were arrested in the G1 stage of the cell cycle and extended periods of their viability, enabling an extended production phase. This would greatly contribute to a more economic industrial process for the production of recombinant proteins. Meanwhile many studies reported that hypothermic cultures on recombinant protein production in rCHO cells were both cell-specific and product-specific. Although the mechanisms regulating these phenotypes during hypothermic culture are pooly understood, these data could be useful for future approaches to enhance the production of recombinant protein in rCHO cells.