치주염 동물모델을 이용한 해조류의 항염증 효과

Anti-inflammatory Effects of Seaweeds Using Periodontitic Animal Model

Objectives: This study investigated the anti-inflammatory activity of four kinds of seaweeds (Ecklonia cava, Saccharina japonica, Undaria pinnatifida, and Gelidium amansii) in the RAW 264.7 cell line and in a mouse model of periodontitis. Methods: The anti-inflammatory activity of prepared ethanol extracts of the seaweeds was screened in the RAW 264.7 cell line. A diet containing 5% of each seaweed was administered to the mice for 2 weeks, followed by lipopolysaccharide (LPS) injection into the periodontal tissue to induce inflammation. Nitric oxide (NO) production was analyzed by the Griess reaction and expression of inflammatory marker proteins was determined by western blotting. Results: All four ethanol extracts inhibited NO production in a concentration-dependent manner without cytotoxicity. In addition, all four seaweed extracts inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions in periodontal tissues of LPS-injected C57BL/6 mice. The mouse group administered U. pinnatifida ethanol extract (UPEE) showed the strongest anti-inflammatory response. Conclusions: The four seaweeds, E. cava, S. japonica, U. pinnatifida, and G. amansii, all exhibited anti-inflammatory activity in both the RAW 264.7 cell line and the LPS-induced periodontitic mouse model. UPEE showed the most potent anti-inflammatory activity. These seaweeds might be promising candidates for alleviating periodontal inflammation. Further research is needed to confirm their effectiveness as preventive agents for periodontal disease.