무지개송어(Oncorhynchus mykiss)정자의 동결보존

Cryopreservation of Spermatozoa in Rainbow Trout(Oncorhynchus mykiss)

이 연구에서는 연어과 어류인 무지개송어(Oncorhynchus mykiss)정자의 동결보존에 알맞은 동결속도, 해동속도에 관하여 진행하였다. 희석액으로는 300 mM Glucose, 20 mM KCl, 20 mM Hepes, pH 7.6을 사용하였으며, 동해방지제로서 dimethyl sulfoxide를 10%의 비율로 사용하였다. 동결속도는 액체질소 표면 2 ㎝ 부근에서 예비동결을 실시한 것이 가장 높은 운동성을 확인할 수 있었다. 해동속도에 따른 정자 운동성은 10℃에서 30초간 해동한 정자의 운동성이 가장 높은 것으로 나타났다.

This experiments was performed to identify the suitable cryoprotectants, freezing rate and, thawing rate for sperm cryopreservation in the rainbow trout, Oncorhynchus mykiss. In cryopreservation experiments, 300 mM glucose solution containing 20 mM KCl, 20 mM Hepes and, pH 7.6 was used as a diluent, and 10% dimethyl sulfoxide (DMSO) was used as a cryoprotectant, and 10 % egg yolk was used as an addictive material. As a results of cryopeservation, when 10 % DMSO was used as a cryoprotectant, sperm motility showed the highest rate at the height of 2 ㎝ away from the liquid nitrogen. Thawing at 10 ℃ for 30 seconds displayed the best result in the thawing rate.