Transcriptional response of heat shock protein 26 on the Pacific abalone Haliotis discus hannai following increased temperature stress
- 전남대학교 수산과학연구소
- 수산과학연구소 논문집
- 제24권 제1호
- 2016.02
- 46 - 51 (6 pages)
Heat shock proteins (HSPs) production in cell is inducible by many physical and chemical stressors, providing adaptive significance for organisms when faced with environmental changes. In this study, we investigated transcriptional expressions of heat shock protein 26 (HSP26) by different temperature stress (20, 22, 24, 26, and 28°C) on Haliotis discus hannai, an important marine gastropod. After 12 h temperature exposure, HSP26 gene expression generally down-regulated in H. discus hannai exposed to all temperatures (p <0.05), in compare to 20°C control. Molecular response of HSP26 also significantly decreased in H. discus hannai exposed to increasing temperature for 48 h. In addition, transcriptional level of HSP26 mRNA was dramatically decreased as 5-fold or more in exposure condition of the relative high temperature (26 and 28°C) for 72 h. The down-regulation observed as temperature-dependent manner. These results suggest sensitive response of HSP26 gene could be used as a molecular marker to estimate stress condition in H. discus hannai abalones following increasing temperature.
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES