세균을 이용한 V 약침의 복귀돌연변이 시험

Bacterial Reverse Mutation Test of V-YAKCHIM

Objectives : This study was designed to determine the mutagenic potential of V-YAKCHIM using histidine requiring Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and tryptophan requiring Escherichia coli(WP2uvrA(pKM101)) strains. Methods : In order to determine the dose levels of the main study, the dose range finding study was conducted. The high dose was selected at 5,000 μg/plate and it was sequentially diluted by the geometric ratio of 4 to 19.5, 78.1, 313 and 1,250 μg/plate. As a result, the growth inhibition and deposition by the test substance was not evident at any dose levels in the absence and presence of metabolic activation. Therefore, the dose levels of the main study were selected as 313, 625, 1,250, 2,500, and 5,000 μg/plate. Also, the negative and positive control groups were used. Results : As a result of the main study, the mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the test substance in the presence and absence of metabolic activation, without dose-related increase. In the positive control group, the number of revertant colonies was markedly increased when compared to that of the negative control group. Conclusions : Based on the results of this study, the test substance, V-YAKCHIM, did not exhibit any indications of mutagenic potential under the conditions of this study.