세균을 이용한 CA약침의 복귀돌연변이 시험

Bacterial Reverse Mutation Test of CA-YAKCHIM

Objectives : The purpose of this study was to determine the mutagenic potential of CA-YAKCHIM using histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli(WP2uvrA(pKM101)) strains in the absence and presence of metabolic activation. Methods : In order to determine the dose levels of the main study, the dose range finding study was performed first. The high dose was selected at 5,000 μg/plate and it was sequentially diluted by the geometric ratio of 4 to 1,250, 313, 78.1, and 19.5 μg/plate. As a result, the growth inhibition by the CA-YAKCHIM was not evident at any dose levels in the absence and presence of metabolic activation. Therefore, the dose levels of the main study were selected as 313, 625, 1,250, 2,500, and 5,000 μg/plate. And the negative (acetone) and positive (sodium azide, 2-Nitrofluorene, 9-Aminoacridine, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide, and 2-Aminoanthracene) control groups were used. Results : The mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the CA-YAKCHIM in the presence and absence of metabolic activation, without dose-related increase. In the positive control group, the number of revertant colonies was markedly increased when compared to that of the negative control group. Conclusions : In this bacterial reverse mutation test, CA-YAKCHIM did not exhibit any indications of mutagenic potential.