LPS로 염증이 유도된 RAW 264.7 대식세포에 대한 B 약침액의 항염증 효과

Anti-inflammatory Activity of B Yakchim Fluid on the LPS-stimulated RAW 264.7 Macrophages

Objectives : The purpose of this study was to investigate the anti-inflammatory activities of B Yackchim fluid. Methods : The total sugar, total reducing sugar, and protein contents in B Yackchim fluid were analyzed. To investigate the anti-inflammatory effects of B Yackchim fluid, nitric oxide (NO), interleukin-2 (IL-2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the LPS-stimulated RAW 264.7 macrophage were measured. Results : B Yackchim fluid consists of 0.12 mg/ml of total sugar, 0.08 mg/ml of total reducing sugar. However, total protein was not detected. B Yackchim fluid showed anti-inflammatory effects by reducing the production of NO, IL-2, IL-6, and TNF-α. However, B Yackchim fluid showed cell cytotoxicity. Conclusions : B Yackchim fluid might have anti-inflammatory effects. However, more studies including efficacy and safety should be needed to use anti-inflammatory agent in Korean medicine.