CSⅡ 약침의 마우스를 이용한 소핵시험

In Vivo Micronucleus Test of CSⅡ-YAKCHIM in Mice

Objectives : This study was designed to determine the potential of CSⅡ-YAKCHIM, manufactured from Brassicae Semen, Raphani Semen, Persicae Semen, and Carthami Fructus extracts, to induce micronuclei in bone marrow cells of mice when the CSⅡ-YAKCHIM was dosed once to ICR mice by intramuscular. Methods : Each of the groups consisted of three doses of CSⅡ-YAKCHIM (0.05, 0.1, and 0.2 mL/animal), positive control (Mitomycin C), and negative control (normal saline). The time to harvest bone marrow cells for the main study was 24 hours. Results : The incidence of micronucleated polychromatic erythrocyte (MNPCE) among polychromatic erythrocyte(PCE) was not significantly increased in the CSⅡ-YAKCHIM-treated groups. Also, the ratio of PCE among total erythrocyte of the CSⅡ-YAKCHIM-treated groups was not significantly different when compared to that of the negative control group. Conclusions : Based on these results, CSⅡ-YAKCHIM did not have any potential to induce micronuclei formation in bone marrow cells of mice under the conditions of this study.