약침 V와 A의 동등성에 대한 연구

Comparative Studies on the Biological Activity of V and A Yakchim

Objectives : The purpose of this study was to investigate the equivalence between A Yakchim (AY) and V Yakchim(VY) through anti-oxidant and anti-inflammatory activity. Methods : In this study, we analyzed the amount of total sugar, reducing sugar, protein, flavonoid and polyphenol of VY and AY. The anti-oxidant activity was determined by assaying the level of radical scavenging activity against 2,2‘-diphenyl-1-picrylhydrazyl (DPPH) and we investigate the anti-inflammatory effect of VY and AY by measuring the production of nitric oxide (NO) in Raw 264.7 cells activated by lipopolysaccharide. Results : VY (10 mg/ml) and AY (10 mg/ml) showed 4.7±1.7% and 61.7±2.1% of DPPH radical scavenging activity, respectively. And VY (1 mg/ml) and AY (1 mg/ml) decreased 17% and 42% of the NO production, respectively. Conclusions : These results suggested the possibility that the anti-oxidant activity and anti-inflammatory activity of AY are better than VY.