주름 개선용 약침 B 개발을 위한 기초 연구

The Basic Study on the Development of B-Yakchim for Improving Wrinkles

Objectives : HO-Yakchim and HN-Yakchim can improve wrinkles. However, these oil-type Yakchims show side effects such as induration or imperfect skin absorption. Therefore, a new Yakchim that can improve wrinkles without causing side effects is required. We developed a new Yakchim B (BY) for wrinkle improvement with a complex herbal extract containing Carthami Fructus, Juglandis Semen Oil, Phellodendri Cortex, Pulsatillae Radix, Sophorae Tonkinensis Radix et Rhizoma, Aucklandiae Radix, and Aquilariae Lignum and investigated the pharmacological activities of BY. Methods : The anti-oxidant effect of BY was investigated by determining its 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity. To investigate the anti-wrinkle activity of BY, its collagenase inhibitory activity was measured. To investigate the skin-lightening effect, the tyrosinase inhibitory activity was measured. In addition, the inhibitory effect of BY on LPS-induced nitric oxide (NO) production in RAW 264.7 cells was investigated and the cell viability of BY on RAW 264.7 cells was investigated using MTT assay. Results : B-Yakchim (10 mg/ml) showed a 52.7±1.0% DPPH radical scavenging rate and 19.4±1.3% collagenase inhibitory activity. However, B-Yakchim (1, 5, and 10 mg/ml) did not show tyrosinase inhibitory activity. B-Yakchim (1 mg/ml) inhibited LPS-induced nitric oxide (NO) production in RAW 264.7 and did not show cell toxicity in the MTT assay. Conclusions : These results suggested that BY has anti-wrinkle, anti-oxidant, and anti-inflammatory activities. These findings could facilitate the development of an anti-wrinkle Yakchim without side effects.