M 약침의 항산화 및 항염 활성에 대한 연구

Anti-oxidant and Anti-inflammatory Effects of M Yakchim

Objectives : We developed new Yakchim M (MY) for activation of brain function and inhibition of inflammation with complex herbal extract including Gastrodiae Rhizoma, Cynanchi Wilfordii Radix, Polygalae Radix, Acori Graminei Rhizoma, Aucklandiae Radix, and Aquilariae Lignum and investigated the pharmacological activities and cell toxicity of MY. Methods : The anti-oxidant effect of MY was investigated by determining 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity. We investigated for the anti-inflammatory effect of MY, LPS-induced nitric oxide (NO) production in RAW 264.7 cells. Also We investigated cell toxicity of MY, cell viability of MY on RAW 264.7 cells using MTT assay. Results : MY showed dose dependent DPPH radical scavenging rate and inhibitory effect of LPS-induced nitric oxide (NO) production in RAW 264.7. and MY (1 mg/ml) did not show cell toxicity in the MTT assay. Conclusions : These results suggested that MY has anti-oxidant, and anti-inflammatory activities. However, more efficacy and safety studies are needed for the development of MY as a medicinal preparation on brain function improvement and anti-inflammation.