Influence of environmental factors on biodegradation of quinalphos by Bacillus thuringiensis
- Venkata Subba Reddy Gangireddygari Rajasekhar Reddy Bontha Bong-Nam Chung In-Sook Cho Ju-Yeon Yoon
- 인간식물환경학회 학술대회
- 2020년 추계학술대회 자료집
- 237 - 237 (1 pages)
The extensive and intensive uses of organophosphorus insecticide-quinalphos in agriculture, pose a health hazard to animals, humans, and environment because of its persistence in the soil and crops. However, there is no much information available on the biodegradation of quinalphos by the soil micro-organisms, which play a significant role in detoxifying pesticides in the environment; so research is initiated in biodegradation of quinalphos. A soil bacterium strain, capable of utilizing quinalphos as its sole source of carbon and energy, was isolated from soil via the enrichment method on minimal salts medium (MSM). On the basis of morphological, biochemical and 16S rRNA gene sequence analysis, the bacterium was identified as to be Bacillus thuringiensis. Bacillus thuringiensis grew on quinalphos with a generation time of 28.38 min or 0.473 h in logarithmic phase. Maximum degradation of quinalphos was observed with an inoculum of 1.0 OD, an optimum pH (6.5-7.5), and an optimum temperature of 35-37°C. Among the additional carbon and nitrogen sources, the carbon source - sodium acetate and nitrogen source - a yeast extract marginally improved the rate of degradation of quinalphos. Display of degradation of quinalphos by B. thuringiensis in liquid culture in the present study indicates the potential of the culture for decontamination of quinalphos in polluted environment sites.