배추 완전장 유전자 과발현 애기장대 형질전환체 활용 내염성 유전자 선발 및 유채 형질전환

Functional Screening of Salt Stress Tolerance Genes Using Transgenic Arabidopsis thaliana Lines Overexpressing Brassica rapa Full-length Genes and Brassica napus Transformation

Given that soil salinity significantly limits plant growth and production in agricultural land, research on salt stress is of particular agricultural relevance. In this study, for the purposes of functional screening of genes involved in salt stress responses, we selected approximately 651 transgenic Arabidopsis lines (157 independent full-length) from a transgenic Arabidopsis population overexpressing full-length Brassica rapa cDNAs. Initial screening indicated that the transgenic lines of 12 genes showed apparent salt tolerance phenotypes when exposed to NaCl at a concentration of 125 mM, among which, two genes (BrATL30 and BrZHD10) were selected for detailed characterization. The T3 progeny of these transgenic lines exhibited accelerated seed germination, often accompanied by faster root growth and higher survival rate, compared with wild-type plants under salt stress. Additionally, in order to examine the agricultural potential of the two selected B. rapa genes, we constructed BrATL30- and BrZHD10-overexpressing Brassica napus transgenic plants (BrATL30-OX and BrZHD10-OX), which showed apparent high salt stress-tolerant phenotypes compared with wild-type plants. Furthermore, we found that the basal expression of several saltand abiotic stress-responsive genes was higher in transgenic plants than in wild-type plants. Taken together, this study will provide two valuable functional genes related to salt stress tolerance.