The PROTECTIVE Effect of White Beech Mushroom(Hypsizygus Marmoreus) Extract against Atopic Dermatitis Skin Lesion Model using NC/Nga Mice

Purpose: This study was carried out to evaluate the protective effects of Hypsizygus marmoreus extract against sodium dodecyl sulfate-induced atopic dermatitis-like skin lesions in vivo. Methods: Six-week-old NC/Nga mice were divided into a control group(Ctrl), atopic dermatitis induction group(ADIG), and H. marmoreus extract feeding group after atopic dermatitis induction(HTFG). After 3 weeks, cannabinoid receptors type 1(CB1) and type 2(CB2), and the orphan G protein-coupled receptor 55(GPR55) levels were observed to verify the modulation of the endocannabinoid system activity, whereas filaggrin and kallikrein-related peptidase(KLK) 7 expression in the stratum corneum and protease activated recep-tor(PAR)-2 expression in the interstitial space of epithelial tissue were observed to verify the inhibition of in-flammation. Furthermore, phosphorylated extracellular signal-related kinase(p-ERK), phosphorylated mamma-lian target of rapamycin(p-mTOR), and E-cadherin levels were studied to verify microenvironmental regulation. Results: Expression of CB1, CB2, and GPR55 was significantly increased in HTFG as compared to that in ADIG. The positive reaction of filaggrin in the stratum corneum was significantly higher in HTFG than in ADIG. KLK7 and PAR-2 positivity in HTFG were significantly reduced as compared to those in ADIG. The p-ERK-positive and p-mTOR-positive reactions in HTFG were significantly reduced as compared to those in ADIG. Conclusion: H. marmoreus extract has a beneficial effect on the epithelium barrier function and can inhibit the progression of atopic dermatitis.