Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis s rhizome and cultivated callus. In EtOAc, BuOH, H₂0separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC50, 35(g), BuOH(RC50, 43(g), H₂0(RC50, 50(g) fraction and MeOH extract(RC50, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC50, 14(g) and α-Tocopherol(RC50, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.