잿빛곰팡이병 추출물을 이용한 순무배양세포의 Indole-3-ylmethyl glucosinolate의 생합성유도와 병원성연구

Elicitation of Indole-3-ylmethyl Glucosinolate Biosynthesis in Turnip Culture Cells and Their Relationship with Plant Resistance to Botrytis cinerea

8종의 잿빛곰팡이병 균주를 순무잎에 접종하여 병반의 크기를확인한 결과 가장 강한 감염력을 보인 ‘포도-01’ 균주와 병반의확산이 가장 적은 ‘오랜지’를 선발하였다. 순무잎이 저항성을 보인 ‘오랜지’균주를 처리한 잎이 감수성을 보인 ‘포도-01’균주를처리한 잎보다 indole-3-ylmethyl glucosinolate (I3M-GLS) 함량이 무처리 보다 2.5배 이상 높았으나 ‘포도-01’ 균주를 처리한 잎에서는 무처리 보다 낮은 함량을 보였다. 균주의 메탄올추출액과 물추출물을 식물배양세포에 처리한 결과 ‘오랜지’균주의 추출물이 ‘포도-01’ 균주의 추출물보다 배양세포의 생장을 더 강하게 억제 한 것으로 나타났는데 ‘오랜지’ 균주의 메타놀및 물 추출물 처리에서 배양세포의 활력은 각각 22.7% 및 16.5% 감소시키는 것으로 나타났다. 한편 ‘오랜지’균주 추출물을 처리한 배양세포에서 I3M-GLS의 생합성이 ‘포도-01’ 균주 추출물보다 현저히 높은 것으로 나타났다. 본 결과로 보아 식물체내에생합성되는 I3M-GLS 함량은 잿빛곰팡이균에 대한 식물세포의저항성과 밀접한 관계가 있는 것으로 판단된다

Two different races of Botryris cinerea were selected by the response of plant leaves to the pathogen infection. Based on lesion size of the pathogen on the leaves, turnip showed susceptible response to ‘Grape-01’ race, and resistant to ‘Orange’ race. Turnip leaves infected with resistant pathogen race, “Orange”, showed significantly higher content of indole-3-ylmethyl glucosinolate (I3M) than those infected with susceptible race, ‘Grape-01’. Contents of I3M in the leaves with resistant ‘Orange’ race was 2.5 times as high as that in uninfected leaves, whereas I3M in the leaves infected with susceptible ‘Grape-01’ race showed lower content than in untreated leaves. Growth of turnip suspension cells was significantly inhibited by the treatment of MeOH extract or water extract of ‘Orange’ race as compared with the treatment of susceptible race, ‘Grape-01’. Treatment of MeOH or water extract from ‘Orange’ race to turnip suspension cells, strongly inhibited cell viability up to 22.7% or 16.5%, respectively. However, plant cells treated with MeOH or water extract from resistant race, ‘Orange’ showed higher I3M content than that from susceptible race, ‘Grape-01’. These results suggest that accumulation and degradation of I3M glucosinolate in turnip cells closely related to the resistance and susceptibility of turnip cells to Botrytis cinerea.